A Dragonfly 3D World plugin written at the Max Planck Florida Institute for Neuroscience. It provides a fast, semi-automatic method for separating dendritic spine heads from their respective spines on a neuron's dendrite. By separating the spine head, this tool allows a neuroscience researcher to quickly and accurately find the volume of a dendritic spine head.
- Preprocessing
- Data preprocessing done automatically and saved to a
.dsbvfile for later use
- Data preprocessing done automatically and saved to a
- Analysis
- Automatically suggests a point to behead with easy human adjustment by dragging a slider, moving the beheading point along the spine
- Visualization
- Optionally visualize annotations labelling synapses for automatic inference of dendrite names
- Optionally visualize the postsynaptic densities (PSDs) segmented to help understand where the synapse is on the dendritic spine
- Output
- Automatically CSV file with the dendritic spine head name and volume
Please view the installation guide
Please view the user guide