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V 0.1.0

📑 Table of Contents

• Introduction
• Features
• Setup and Installation
• Contributors
• Contacts

🧬 Introduction

RFLP Finder is a comprehensive tool designed to streamline the process of analyzing restriction fragment length polymorphisms (RFLPs). By integrating genome handling, SNP extraction, enzyme pattern recognition, and RFLP analysis and PCR-RLFP preparing, this software is ideal for researchers in genomics and molecular biology.

The tool enables:

• Automated or manual genome management.
• Identification of SNPs and enzyme recognition patterns.
• Primer design and RFLP analysis with customizable parameters using Primer3.
• Flexible workflow options, including a guided mode and an autopilot mode.

Key Features

• Genome Management: Download genomes automatically or use your own pre-downloaded files.
• SNP Analysis: Extract and process flanking sequences around specified SNPs.
• Enzyme Recognition: Identify restriction enzyme recognition sites with high precision.
• Customizable RFLP Analysis: Fine-tune primer and PCR parameters for tailored results.
• Comprehensive Cleanup Tool: Remove past results and optionally delete downloaded genomes.
• Autopilot Mode: Automate the entire process using a configuration file.

🔧 Setup and Installation

Requirements

• Python v3.7+

Installation Instructions

# Clone this repository
git clone https://github.com/Kubilay-Meydan/RFLP-Finder.git
cd RFLP-Finder

# Install required Python libraries
pip install -r requirements.txt

# Launch the pipeline
python main.py

👥 Contributors

This project was developed by:

• Kubilay Meydan

🗨️ Contacts

For questions or suggestions, feel free to reach out:

• Email: kubilay.meydan2002@gmail.com
• GitHub: Kubilay-Meydan

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