Barcodes - "Warning! None of the annotated barcodes were detected."

[EvlampiaPar]

Hello guys,
I have a question regarding the BC (barcodes).

In my job-UMIs.out i have among other information, the below:

Filtering...
Tue Feb 11 11:33:42 CET 2025
[1] "Warning! None of the annotated barcodes were detected."
[1] "Continuing with top 100 barcodes instead..."
[1] "358627 reads were assigned to barcodes that do not correspond to intact cells."
[1] "Found 659 daughter barcodes that can be binned into 60 parent barcodes."
[1] "Binned barcodes correspond to 325242 reads."

I gave as input list for yaml file, a txt file without header with a list of my 8-mer indices, like that:
TAAGGCGA
ATAGAGAG
TAAGGCGA
AGAGGATA
TAAGGCGA
TATGCAGT
TAAGGCGA

I listed my indices in reverse complement strand. So total i had 120 indices (since it is paired-end).

I had 60 cells for sequencing.

Also, to add, that i use concatenated sequencing reads (R1s and R2s) and concatenated index reads (I1s and I2s) fastq.gz files.

Why in the output i get the message that ""Warning! None of the annotated barcodes were detected." ?

Happy to hear your feedback! Thank you!