Skip to content

Questions on loading fastq.gz, and output.sam #111

@rl4940

Description

@rl4940

Hi Fritz team!

Thank you so much for developing this aligner. Currently I am using NGMLR along with minimap2, and I have 2 main questions regarding this:
below is my current code:

#### running ngmlr ####
ngmlr -t 20 -r $ref -q test_pipe1_standard/hg002head1.fastq.gz -o hg002.sam -x ont

### sorting and indexing the bam file ####
samtools view -b --threads 10 hg002.sam > hg002_out.bam
samtools sort hg002_out.bam -o hg002_out.sorted.bam
samtools index -M --threads 10 hg002_out.sorted.bam

Question 1: how to load multiple fastq.gz files into NGMLR? e.g. HG002 on GIAB has 3 fastq.gz files, so how to load them in?
Question 2: the output from NGMLR is SAM format, to eliminate unnecessary files, is there a way to re-set it into BAM output? if so, is there a way to auto-index the BAM file in NGMLR just like --write-index in minimap2?

Thanks!
R.L.

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions