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downsampling_bam_paired_snakemake

A snakemake to downsample reads from a bam file, following GATK best practices.

This repository contains all the folders needed to run the snakemake. logs/cluster/ currently contains a placeholder file because GitHub does not allow empty folders, but after copying this repository structure to the server, this placeholder file can be removed from the server.

To run:

  1. update config/samples.yaml and config/config.yaml as per the directions in those files

  2. load all modules listed in downsampling_bam_paired.snakefile

  3. verify all paths are correct for your application

  4. launch snakemake from directory containing the snakemake file with command referenced downsampling_bam_paired.snakefile (with -np) for a dry run which will look for errors and list out all necessary jobs. If no errors and jobs look correct

  5. relaunch snakemake with command referenced in downsampling_bam_paired.snakefile without -np

  6. snakemake steps will be launched as jobs to the cluster at the Fred Hutchinson Cancer Research Center.