diff --git a/environment-files/q2-kmerizer-qiime2-amplicon-2025.10.yml b/environment-files/q2-kmerizer-qiime2-amplicon-2025.10.yml
new file mode 100644
index 0000000..2eda26e
--- /dev/null
+++ b/environment-files/q2-kmerizer-qiime2-amplicon-2025.10.yml
@@ -0,0 +1,11 @@
+channels:
+- https://packages.qiime2.org/qiime2/2025.10/amplicon/passed
+- conda-forge
+- bioconda
+dependencies:
+  - qiime2-amplicon
+  - numpy
+  - scikit-learn
+  - pip
+  - pip:
+     - "q2_kmerizer@git+https://github.com/bokulich-lab/q2-kmerizer.git@main"