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fix URLs on getting-started examples
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docs/getting-started/index.md

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@@ -38,7 +38,7 @@ their counts.
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Load this example up with `countess_gui example_1.ini`.
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[![Example 1 Image 0](../../../countess-demo/example_1/img/example_1_0.png)](../../../countess-demo/example_1/img/example_1_0.png)
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[![Example 1 Image 0](/countess-demo/example_1/img/example_1_0.png)](/countess-demo/example_1/img/example_1_0.png)
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There are five nodes in this CountESS pipeline, each of which
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transforms the data and then passes it to the next node.
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Our first step just reads these two files in.
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[![Example 1 Image 1](../../../countess-demo/example_1/img/example_1_1.png)](../../../countess-demo/example_1/img/example_1_1.png)
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[![Example 1 Image 1](/countess-demo/example_1/img/example_1_1.png)](/countess-demo/example_1/img/example_1_1.png)
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* Sequence data can also be loaded from other file formats such
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as FASTQ, see [Example 3](#example-3-fastq-and-vamp-seq)
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Next we want to count how many times each sequence appears at each
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time point.
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[![Example 1 Image 2](../../../countess-demo/example_1/img/example_1_2.png)](../../../countess-demo/example_1/img/example_1_2.png)
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[![Example 1 Image 2](/countess-demo/example_1/img/example_1_2.png)](/countess-demo/example_1/img/example_1_2.png)
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The [Group By](../included-plugins/#group-by) tool lets us specify some columns to index by.
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If no other operations are selected, it will count the number of
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To do this, we use the [Pivot Tool](../included-plugins/#pivot-tool):
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[![Example 1 Image 3](../../../countess-demo/example_1/img/example_1_3.png)](../../../countess-demo/example_1/img/example_1_3.png)
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[![Example 1 Image 3](/countess-demo/example_1/img/example_1_3.png)](/countess-demo/example_1/img/example_1_3.png)
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The distinct values of the "Pivot" column(s) are used to expand the
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data in the "Expand" column(s) into new columns.
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two different time points. We want to calculate scores from the counts
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by dividing one by the other:
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[![Example 1 Image 4](../../../countess-demo/example_1/img/example_1_4.png)](../../../countess-demo/example_1/img/example_1_4.png)
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[![Example 1 Image 4](/countess-demo/example_1/img/example_1_4.png)](/countess-demo/example_1/img/example_1_4.png)
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The [Python Code](../included-plugins/#python) tool lets us write small expressions in Python and
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apply them to each row of the table. In this case we're calculating
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out somewhere. The [CSV Save](../included-plugins/#csv-writer)
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tool lets us save our output in a CSV file for easy use elsewhere.
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[![Example 1 Image 5](../../../countess-demo/example_1/img/example_1_5.png)](../../../countess-demo/example_1/img/example_1_5.png)
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[![Example 1 Image 5](/countess-demo/example_1/img/example_1_5.png)](/countess-demo/example_1/img/example_1_5.png)
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The output ends up looking like:
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First, we modify our sequence reading and grouping steps to rename the sequence column to `barcode`,
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for clarity.
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[![Example 2 Image 1](../../../countess-demo/example_2/img/example_2_1.png)](../../../countess-demo/example_2/img/example_2_1.png)
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[![Example 2 Image 2](../../../countess-demo/example_2/img/example_2_2.png)](../../../countess-demo/example_2/img/example_2_2.png)
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[![Example 2 Image 1](/countess-demo/example_2/img/example_2_1.png)](/countess-demo/example_2/img/example_2_1.png)
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[![Example 2 Image 2](/countess-demo/example_2/img/example_2_2.png)](/countess-demo/example_2/img/example_2_2.png)
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Second, we add a new node to read the barcode map using the
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[CSV Loader](../included-plugins/#csv-reader):
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[![Example 2 Image 3](../../../countess-demo/example_2/img/example_2_3.png)](../../../countess-demo/example_2/img/example_2_3.png)
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[![Example 2 Image 3](/countess-demo/example_2/img/example_2_3.png)](/countess-demo/example_2/img/example_2_3.png)
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### Joining
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Now we add in a [Join](../included-plugins/#join) tool, which takes two inputs and joins them.
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[![Example 2 Image 4](../../../countess-demo/example_2/img/example_2_4.png)](../../../countess-demo/example_2/img/example_2_4.png)
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[![Example 2 Image 4](/countess-demo/example_2/img/example_2_4.png)](/countess-demo/example_2/img/example_2_4.png)
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* Note that while there were 1000 distinct barcodes, there are only 357 distinct
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sequences. Some barcodes represent duplicate variants.
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plugin lets us compare a sequence to a reference sequence and extract
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DNA and Protein variants in [HGVS](https://hgvs-nomenclature.org/stable/) format.
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[![Example 2 Image 5](../../../countess-demo/example_2/img/example_2_5.png)](../../../countess-demo/example_2/img/example_2_5.png)
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[![Example 2 Image 5](/countess-demo/example_2/img/example_2_5.png)](/countess-demo/example_2/img/example_2_5.png)
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We add a Variant Translator to our project, and configure it with
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our known reference sequence:
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pivot, score and write to CSV steps as before, but duplicated for both DNA and Protein variants.
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<!--
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[![Example 2 Image 6](../../../countess-demo/example_2/img/example_2_6.png)](../../../countess-demo/example_2/img/example_2_6.png)
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[![Example 2 Image 9](../../../countess-demo/example_2/img/example_2_9.png)](../../../countess-demo/example_2/img/example_2_9.png)
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[![Example 2 Image 7](../../../countess-demo/example_2/img/example_2_7.png)](../../../countess-demo/example_2/img/example_2_7.png)
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[![Example 2 Image 10](../../../countess-demo/example_2/img/example_2_10.png)](../../../countess-demo/example_2/img/example_2_10.png)
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[![Example 2 Image 6](/countess-demo/example_2/img/example_2_6.png)](/countess-demo/example_2/img/example_2_6.png)
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[![Example 2 Image 9](/countess-demo/example_2/img/example_2_9.png)](/countess-demo/example_2/img/example_2_9.png)
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[![Example 2 Image 7](/countess-demo/example_2/img/example_2_7.png)](/countess-demo/example_2/img/example_2_7.png)
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[![Example 2 Image 10](/countess-demo/example_2/img/example_2_10.png)](/countess-demo/example_2/img/example_2_10.png)
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-->
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[![Example 2 Image 8](../../../countess-demo/example_2/img/example_2_8.png)](../../../countess-demo/example_2/img/example_2_8.png)
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[![Example 2 Image 11](../../../countess-demo/example_2/img/example_2_11.png)](../../../countess-demo/example_2/img/example_2_11.png)
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[![Example 2 Image 8](/countess-demo/example_2/img/example_2_8.png)](/countess-demo/example_2/img/example_2_8.png)
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[![Example 2 Image 11](/countess-demo/example_2/img/example_2_11.png)](/countess-demo/example_2/img/example_2_11.png)
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## Example 3: FASTQ
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in the FASTQ Loader which will add an extra `filename` column in to the
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dataframe:
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[![Example 3 Image 1](../../../countess-demo/example_3/img/example_3_1.png)](../../../countess-demo/example_3/img/example_3_1.png)
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[![Example 3 Image 1](/countess-demo/example_3/img/example_3_1.png)](/countess-demo/example_3/img/example_3_1.png)
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We can then use the [Regex Tool](../included-plugins/#regex-tool) to break this filename down into its
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useful parts, extracting `bin` and `rep` columns from the filename:
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[![Example 3 Image 2](../../../countess-demo/example_3/img/example_3_2.png)](../../../countess-demo/example_3/img/example_3_2.png)
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[![Example 3 Image 2](/countess-demo/example_3/img/example_3_2.png)](/countess-demo/example_3/img/example_3_2.png)
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<!--
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[![Example 3 Image 3](../../../countess-demo/example_3/img/example_3_3.png)](../../../countess-demo/example_3/img/example_3_3.png)
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[![Example 3 Image 3](/countess-demo/example_3/img/example_3_3.png)](/countess-demo/example_3/img/example_3_3.png)
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-->
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In the previous example, we chose to insert the [Variant Translator](../included-plugins/#variant-translator)
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after the [Join](../included-plugins/#join) for clarity, but when
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we have many more sequences to call it is more efficient to call the variant
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once for each barcode rather than once for each sequence:
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[![Example 3 Image 4](../../../countess-demo/example_3/img/example_3_4.png)](../../../countess-demo/example_3/img/example_3_4.png)
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[![Example 3 Image 4](/countess-demo/example_3/img/example_3_4.png)](/countess-demo/example_3/img/example_3_4.png)
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<!--
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[![Example 3 Image 5](../../../countess-demo/example_3/img/example_3_5.png)](../../../countess-demo/example_3/img/example_3_5.png)
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[![Example 3 Image 5](/countess-demo/example_3/img/example_3_5.png)](/countess-demo/example_3/img/example_3_5.png)
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-->
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The two data sources are joined as before. We can then [pivot](../included-plugins/#pivot-tool)
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on `bin`, giving us columns `count__bin_1`, `count__bin_2`, `count__bin_3` and `count__bin_4`:
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[![Example 3 Image 6](../../../countess-demo/example_3/img/example_3_6.png)](../../../countess-demo/example_3/img/example_3_6.png)
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[![Example 3 Image 6](/countess-demo/example_3/img/example_3_6.png)](/countess-demo/example_3/img/example_3_6.png)
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## Example 4: External Metadata
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First we load up the metadata file, splitting the columns using
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"TAB" characters:
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[![Example 4 Image 1](../../../countess-demo/example_4/img/example_4_1.png)](../../../countess-demo/example_4/img/example_4_1.png)
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[![Example 4 Image 1](/countess-demo/example_4/img/example_4_1.png)](/countess-demo/example_4/img/example_4_1.png)
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Then we load up the six data files, remembering to select the
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"Filename Column?" option to get the filename as a column:
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[![Example 4 Image 2](../../../countess-demo/example_4/img/example_4_2.png)](../../../countess-demo/example_4/img/example_4_2.png)
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[![Example 4 Image 2](/countess-demo/example_4/img/example_4_2.png)](/countess-demo/example_4/img/example_4_2.png)
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Then we can join the two together using the `filename` column
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in each:
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[![Example 4 Image 3](../../../countess-demo/example_4/img/example_4_3.png)](../../../countess-demo/example_4/img/example_4_3.png)
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[![Example 4 Image 3](/countess-demo/example_4/img/example_4_3.png)](/countess-demo/example_4/img/example_4_3.png)
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# Further Examples
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