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I am currently attempting to run alevin-fry to quantify some snRNA-seq data in USA mode with bootstraps. From my understanding, USA-mode should be allowed to be done with bootstrapping, as the bootstrap flag is meaningful with the cr-like-em quantification method, and that method uses USA mode (https://alevin-fry.readthedocs.io/en/latest/quant.html).
However, when I am trying to run this below script, i get the following error. I would appreciate any guidance and clarification!
alevin-fry quant \
-t 6 \
-i $a/alevin_fry/alevinfry_unfiltered_pl_gencode_quants_noforcecells/${c}quant \
-o $a/alevin_fry/alevinfry_unfiltered_pl_gencode_quants_res_noforcecells/${c}quant \
--tg-map $a/alevin_fry/salmon_index_files/transcriptome_splici_fl_gencode85/transcriptome_splici_fl85_t2g_3col.tsv \
-b 30 \
--resolution cr-like-em \
--use-mtx
ERROR: assertion left == right failed: currently USA-mode (all-in-one unspliced/spliced/ambiguous) analysis cannot be used with bootstrapping.
left: 30
right: 0
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